Figure 2.

(a) Binding of isotype-specific polyclonal phage mixtures obtained after four rounds of affinity selection to immobilized gliadin (400 ng/well). A total of 10¹⁰ phagemid particles were serially diluted 1 : 1 throughout an enzyme-linked immunosorbent assay (ELISA) plate and visualized with horseradish peroxidase (HRP)-labelled anti-M13 monoclonal antibody (mAb). The first four dilutions for each isotype are shown. As background (BG), phagemid particles rescued from the original, unselected libraries were used. (b) Binding of isotype-specific polyclonal phage mixtures (as in panel a) to decreasing amounts of coated gliadin and constant phage load (10¹⁰ phagemid particles/well).