Figure 3.

Interleukin-10 (IL-10)-treated dendritic cells (DC) are responsive to lipopolysaccharide (LPS)-induced maturation. DC were generated from murine bone marrow (BM) cells by incubation with either granulocyte–macrophage colony-stimulating factor (GM-CSF) or GM-CSF + IL-10, until day 8 of culture. Then, the cells were washed and stimulated overnight with LPS or with the same cytokines until day 9 of culture. (a) Flow cytometric analysis of major histocompatibility complex (MHC) class II expression of CD11c⁺ DC stimulated with (bold line) or without (solid line) LPS. (b) Purified C57Bl/6 CD4⁺ T cells were stimulated with graded numbers of BALB/c DC and proliferation was analysed after 4 days by measuring the incorporation of [³H]thymidine.