Figure 4.

Selective expression of regulated on activation, normal, T-cell expressed, and secreted (RANTES) by germinal centre (GC) B-cell subsets. (a) Western blot analysis depicting the selective expression of RANTES by IgD⁻ CD38⁺ GC B cells. (b) Southern blot experiment of a RANTES-specific reverse transcription–polymerase chain reaction (RT–PCR), using total RNA from 500 cells of each of the five mature B-cell subsets. The numbers in the figure correspond to the respective Bm1 to Bm5 subsets. Again, RT–PCR amplification of the immunoglobulin heavy chain (IgHV) was used as an internal control for the RNA content of each subset. The results are autoradiographs of the RANTES hybridization reaction and ethidium bromide gel staining of the IgHV RT–PCR results, respectively.