Table 3.

Cytokine response of 5(6)-carboxyfluorescein diacetate N-succinimidyl ester-negative (CFSE⁻) β3 cells

		Intracellular cytokine staining
		IFN-γ		TNF-α
Exp.	Recipient mice	No peptide	With peptide	No peptide	With peptide
1	B10.A	0·82 ± 0·42*	0·51 ± 0·10	3·20 ± 0·61	2·73 ± 0·87
	hi-arr-β-gal	1·85 ± 1·19	2·66 ± 0·74	2·41 ± 1·28	5·29 ± 1·26
		NS†	0·033	NS	0·020
2	B10.A	1·25 ± 1·06	2·16 ± 1·77	4·38 ± 0·68	3·65 ± 0·62s
	GFAP-β-gal	2·10 ± 0·89	3·09 ± 2·19	3·01 ± 1·50	2·69 ± 1·56
		NS	NS	NS	NS

^*The percentage of total CD8⁺ Vβ8⁺ from the cytokine⁺ CFSE⁻ region (see Fig. 6a–6f, upper left region).

^†Calculated using the t-test; cells were recovered from B10.A versus hi-arr-β-gal mice and from B10.A versus GFAP-β-gal mice.

IFN-γ, interferon-γ; NS, not significant; TNF-α, tumour necrosis factor-α.