Figure 2.

Constitutive transcription of IFN-α, IFN-β, IFN-γ, IL-1β, IL-2, TGF-β₄, MHC IA and β₂M in unstimulated thymocytes. Transcript is quantified as 40–C_t and standardized against a 28S RNA standard. Significant differences in measured transcript are indicated by a star above the sample. (a) Transcript levels measured at E14 (unshaded), E18 (black shading) and D1 (grey shading). (b) Transcript levels in CD4⁺ TCR⁺ (unshaded), CD8⁺ TCR⁺ (light grey), CD4⁺ TCR⁻ (black) and CD8⁺ TCR⁻ (dark grey) subsets at E18.