Table 1.
Real-time quantitative RT-PCR probes and primers
| RNA target | Probe/primer sequence | Accession number | Exon boundaries* | |
|---|---|---|---|---|
| 28S | Probe | (FAM)-AGGACCGCTACGGACCTCCACCA-(TAMRA) | ||
| F | GGCGAAGCCAGAGGAAACT | |||
| R | GACGACCGATTTGCACGTC | |||
| IFN-α | Probe | (FAM)-CTCAACCGGATCCACCGCTACACC-(TAMRA) | U07868 | – |
| F | GACAGCCAACGCCAAAGC | |||
| R | GTCGCTGCTGTCCAAGCATT | |||
| IFN-β | Probe | (FAM)-TTAGCAGCCCACACACTCCAAAACACTG-(TAMRA) | X92479 | – |
| F | CCTCCAACACCTCTTCAACATG | |||
| R | TGGCGTGCGGTCAAT | |||
| IFN-γ | Probe | (FAM)-TGGCCAAGCTCCCGATGAACGA-(TAMRA) | Y07922 | 3/4 |
| F | GTGAAGAAGGTGAAAGATATCATGGA | |||
| R | GCTTTGCGCTGGATTCTCA | |||
| IL-1β | Probe | (FAM)-CCACACTGCAGCTGGAGGAAGCC-(TAMRA) | AJ245728 | 5/6 |
| F | GCTCTACATGTCGTGTGTGATGAG | |||
| R | TGTCGATGTCCCGCATGA | |||
| IL-2 | Probe | (FAM)-ACTGAGACCCAGGAGTGCACCCAGC-(TAMRA) | AJ009800 | 2/3 |
| F | TTGGAAAATATCAAGAACAAGATTCATC | |||
| R | TCCCAGGTAACACTGCAGAGTTT | |||
| TGF-β4 | Probe | (FAM)-ACCCAAAGGTTATATGGCCAACTTCTGCAT-(TAMRA) | M31160 | 6/7 |
| F | AGGATCTGCAGTGGAAGTGGAT | |||
| R | CCCCGGGTTGTGTTGGT | |||
| β2M | Probe | (FAM)-TCTTCAGACCGTGCTCATCCCGG-(TAMRA) | Z48921 | 2/3/4 |
| F | CTACAAGTGGGATCCCGAGTTC | |||
| R | TCATTTCAACTTGGAATGCAGAA | |||
| MHC IA | Probe | (FAM)-ACCGGCGGGTCTCACACAGTGC-(TAMRA) | M84766 | 2/3 |
| F | CAGCGGCGCTACAACCA | |||
| R | GATGTCACAGCCGTACATCCA |
*
There are 10 intronless IFN-α genes, with a high level of sequence identity, and a single intronless IFN-β gene.