Table 2.

Optimized primer concentrations, slope of the regression line and R² statistics for all real-time, quantitative RT-PCR assays

Target	Primer concn (μm)	ΔRn significance threshold	Ct	R2	Gradient
IFN-α	0·1	0·03	15–40	0·9898	3·95
IFN-β	1·0	0·02	15–40	0·9941	3·016
IFN-γ	0·6	0·03	15–40	0·9995	3·135
IL-1β	0·4	0·02	15–40	0·9999	3·408
IL-2	0·6	0·02	15–40	0·989	2·748
TGF-β4	0·1	0·04	15–40	0·9893	3·589
β2M	1·0	0·01	15–40	0·9959	3·442
MHC IA	1·0	0·01	15–40	0·9916	3·213

ΔR_n = change in the reporter dye; C_t = threshold cycle value, the cycle at which the change in the reporter dye levels detected passes the ΔR_n; R² = coefficient of regression.