Table 2.
Effect of LPS on expression of adhesion molecules on human monocytes and lymphocytes*
Monocytes | Lymphocytes | |||||
---|---|---|---|---|---|---|
Molecules | Control | LPS | P-value† | Control | LPS | P-value† |
ICAM-1 | 315 ± 41 | 857 ± 60 | P < 0·01 | 11 ± 1 | 17 ± 2 | NS |
CD14 | 870 ± 54 | 1087 ± 95 | P < 0·01 | 10 ± 5 | 5 ± 1 | NS |
CD18 | 640 ± 88 | 525 ± 66 | NS | 152 ± 20 | 169 ± 20 | NS |
CD11a | 404 ± 52 | 343 ± 33 | NS | 175 ± 7 | 195 ± 32 | NS |
CD11b | 530 ± 90 | 514 ± 105 | NS | 50 ± 16 | 63 ± 19 | NS |
CD11c | 291 ± 56 | 290 ± 29 | NS | 7 ± 1 | 9 ± 1 | NS |
CD29 | 703 ± 39 | 625 ± 78 | NS | 86 ± 11 | 91 ± 15 | NS |
CD49d | 50 ± 6 | 43 ± 4 | NS | 40 ± 3 | 39 ± 2 | NS |
CD49e | 337 ± 9 | 277 ± 32 | NS | 19 ± 3 | 18 ± 3 | NS |
ICAM-2 | 101 ± 8 | 109 ± 22 | NS | 53 ± 2 | 54 ± 3 | NS |
ICAM-3 | 191 ± 28 | 178 ± 33 | NS | 106 ± 5 | 105 ± 7 | NS |
ALCAM | 107 ± 12 | 82 ± 6 | NS | 11 ± 1 | 11 ± 1 | NS |
PBMC were stimulated with LPS (1 µg/ml). The expression of the various adhesion molecules was assessed after 24 hr of incubation. Data represent the mean fluorescence ± SEM of four separate experiments.
NS, not significant.