Figure 6.
Antigen dose, type of antigen-presenting cell (APC) and time of differentiation contribute to T helper 1 (Th1)/T helper 2 (Th2) cytokine production. A total of 3 × 105 syngeneic naive T cells/well were incubated with dendritic cells (DCs) or macrophage (Mφ) (3 × 103/well), pretreated with mitomycin C and different doses of toxic shock syndrome toxin-1 (TSST-1). After 3 or 7 days, cells were assayed for the production of interferon-γ (IFN-γ) and interleukin-4 (IL-4). Data shown are the average from four experiments (a) or dot-plots of intracellular IL-4 and IFN-γ expressed by Vβ2+ cells stimulated with a TSST-1 concentration of 10 ng/ml (b).