Table 1.

IFNγ⁺ CFSE^low CD8⁺ T cell frequencies after stimulation with gag peptides

Patient	6-d culture in medium	6-d culture in peptides	6-d culture in αhDEC-p24
	6-h restim in p24 peptides		6-h restim in peptides	6-h restim in pool I	6-h restim in pool II	6-h restim in pool III	6-h restim in pool IV	6-h restim in pool V
LTNP
LB01 PBMC	0.02	3.1	1.4	0.25	0.34	1	0.38	0.29
LB01 DC/T	0.12	12	6.3	0.97	0.92	6.1	ND	ND
LB02 PBMC	0.04	6.3	3.8	3.2	0.07	0.14	0.64	0.11
LB03 DC/T	0.04	17	2.6	0.54	0	2.0	0.88	0.03
LB04 PBMC	0.02	16	2.1	1.5	0.13	0.27	0.36	0.37
LB04 DC/T	1.52	34.7	43.3	33.7	1.51	5.87	1.58	4.02
LB05 DC/T	0.55	37.6	26.4	1.27	0.45	16.3	19.1	0.44
LB06 PBMC	0	9.73	6.09	1.8	1.38	4.94	1.8	1.58
Chronic
LB07exp1 PBMC	0.02	4.6	2.8	0.28	0.83	1.2	0.54	3.7
LB07 DC/T	0.23	11	3.4	1.2	1.5	3.9	1.4	4.4
LB07exp2 PBMC	0.17	3.9	1.7	0.25	0.14	0.61	0.36	1.3
LB08 DC/T	0.18	4.1	1.5	ND	ND	0.21	0.46	1.7
LB09 PBMC	0.19	4.9	2.8	0.47	1.4	0.39	1.4	0.45
LB10 PBMC	0.022	1.28	1.22	0.09	1.42	0.09	0.09	0.05
LB10 DC/T	1.04	13.6	1.63	0.41	1.32	0.3	0.28	0.21
LB11 PBMC	0.006	10.6	5.96	0.39	5.22	0.55	1.06	0.57

Summary of the frequencies of IFNγ⁺ CFSE ^low CD8⁺ cells obtained from either PBMCs or DC/T cell cocultures stimulated for 6–7 days with medium, p24 peptides, and αDEC p24. The samples were then restimulated for 6–8 h with p24 peptides or with the individual peptide pools (see Materials and Methods). The bold data represent pools of peptides that yield responses that are three times more than the background of nonstimulated cells. LTNP, long-term nonprogressor.