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α-(1,3)-Glucan reduces TNFα production by phagocytic cells in response to Histoplasma yeasts. ELISA-based quantitation of TNFα produced by P388D1 phagocytic cells in response to 3-h infection by live Histoplasma yeast shows increased TNFα stimulation by yeasts that lack the α-(1,3)-glucan polysaccharide compared with yeasts surrounded by α-(1,3)-glucan. All results represent data obtained from triplicate infections, with error bars corresponding to the SD.
