Fig. 3.

Analysis of mutants allelic to vrs1. (A) Lesions at Vrs1 detected in 48 mutants. Arrows pointing down indicate amino acid substitutions, arrows pointing up with a solid line indicate new stop codons, and the three arrows pointing up with a broken line indicate single-nucleotide substitutions in the introns with a changed splicing. The arrowheads and horizontal broken lines indicate deletions, in which five mutants have a partial deletion and seven mutants have a complete deletion of Vrs1. (B) RT-PCR analysis of nine mutant lines (including a New Golden mutant, NG M13) that did not show any lesions on the Vrs1. Four two-rowed cultivars and a deletion mutant (hex-v.3) were included as positive and negative controls, respectively. NG, New Golden. Total RNA was extracted from immature inflorescences 2–3 mm long.