Fig. 4.

The AHR is activated by NaOCl-modified LDL. (A) LDL, BSA, and apoB (all 0.2 mg/ml in PBS) were preincubated in the presence or absence of 100 μM NaOCl for 1 h at 37°C. After extensive dialysis of each sample, H1L6.1c3 cells (n = 4) were treated with 0.1 mg/ml of each sample in serum-free DMEM for 18 h. Statistical comparisons were performed between each NaOCl-modified substrate and its untreated control. (B) LDL was preincubated with the indicated concentration of NaOCl for 1 h at 37°C; a control sample of LDL was incubated without NaOCl. After extensive dialysis, each LDL sample (0.1 mg/ml) was used to treat H1L6.1c3 cells (n = 4) for 18 h. ∗∗∗, P < 0.001.