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Vγ and Vδ repertoires of γδ T cells in persistently infected kidneys after intrarenal injection with L. monocytogenes. The lymphocytes from kidneys were prepared from the mice intrarenally inoculated with L. monocytogenes on day 28 of infection. γδ T cells were purified using MACS. The expression of Vγ and Vδ genes was analysed by RT–PCR as described in Materials and Methods.
