Figure 3.
Time-dependent increases in the adherence of neutrophils to cartilage and activity of PI3-K. Neutrophils (3 × 107 for kinase activity or 6 × 106 51Cr-chromate-labelled for adherence) were incubated with unlabelled cartilage for up to 150 min. The tubes were gently inverted every 30 min to ensure adequate interaction between neutrophils and cartilage. At the end of the incubation period, the pieces of cartilage were washed and the number of adherent neutrophils (a) and PI3-K activity (b) were determined as described in the Materials and Methods. Results are means ±SEM of two experiments (a) performed in triplicates, or representative of three experiments (b) using cells from different donors. The numbers above (b) represent kinase activities, expressed as percentage of the maximum level (150 min in the absence of TNF-α), after deduction of basal kinase activity at 0 min.