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Suppression of superoxide production and release of β-glucuronidase by wortmannin. Cells were pretreated with wortmannin for 15 min before being stimulated with fMLP (100 nm) or TNF-α (1000 U/ml). The production of superoxide and release of β-glucuronidase were assessed as described in the Materials and Methods. Results, means ±SEM of three determinations, are representative of three (c) or four (a, b) experiments, each using cells from a different donor.
