Figure 2.

Stimulation index and cytokine production levels in splenocyte cultures. Splenocytes from each mouse were culture for 5 days. Incorporation of [³H]thymidine was measured in a β-scintillation counter. The stimulation indices of control mice (□) and RT mice (▪) were calculated as a ratio of counts per minute (c.p.m.) in stimulated wells/c.p.m. in control wells. IFN-γ and IL-10 quantification of control mice (open bars) and of RST mice (filled bars) were performed on supernatants using ELISA. After culture with TeNT, IFN-γ and IL-10 were undetectable on day 5 in both groups, and IFN-γ was undetectable on day 19 in the RST group. Results with either TeNT (a) or Con A (b) are expressed as mean ± SE. *P < 0·05; **P < 0·001.