Table 2.
TRAP-deficient | Wild-type | |
---|---|---|
Basal | 3·12 ± 0·87** | 0·73 ± 0·26 |
LPS | 9·69 ± 1·3* | 4·65 ± 0·59 |
IFN-γ | 8·25 ± 1·85 | 4·56 ± 0·85 |
Resident peritoneal Mφ were isolated and stimulated alone or with lipopolysaccharide (LPS) (1 µg/106 cells) or interferon-γ (IFN-γ) (100 U/106 cells) for 48 hr. Sixty minutes before harvesting, cells were stimulated with PMA (1 µg/106 cells) and ferricytochrome c was added (80 µm). Superoxide, determined as described in the Materials and Methods, is shown as µmol of cytochrome c reduced/106 cells/hr. Results are representative of two separate experiments, each using 10 determinations for each condition, and are expressed as mean±SEM.
P < 0·01;
P < 0·05.