Figure 4.

HgCl₂-induced FN adhesion of Jurkat T cells is mediated via VLA-4 and VLA-5. Jurkat T cells were preincubated with control IgG1, 4B4 (anti-CD29), HP2/1 (anti-CD49d), SAM-1 (anti-CD49e), or HP2/1 plus SAM-1. Subsequently, FN adhesion was assessed in the presence or absence of HgCl₂ (2·5 µm), PMA (50 ng/ml), or MnCl₂ (500 µm), as indicated. In separate experiments, optimal blocking concentrations of the mAb were determined, in which SAM-1 was titrated in the presence of HP2/1, and vice versa. Data represent three similar experiments.