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Selective up-regulation of GATA-3 gene in human Th2 clones after TCR activation. One human Th1 (C21.9.4) and one Th2 (C22.5.4) clones were activated either with anti-CD3 mAb (5 µg/ml) coated on plate or with PI. To analyse the GATA-3 expression kinetics, the cells were harvested at different time points (2, 6, 12 hr) after anti-CD3 activation. GATA-3 and cytokine–receptor gene expression were examined by RT–PCR. β-actin PCR (30 cycles) is shown as control.
