Figure 6.
Heat-killed M. vaccae as a mucosal adjuvant. C57BL/6J mice were immunized intranasally with (a) OVA (b) 500 µg heat-killed M. vaccae, or (c) OVA mixed with 500 µg heat-killed M. vaccae. Spleens were removed 10 days later and splenocytes were stimulated in vitro with E.G7-OVA cells for 6 days. The cytotoxic activities were measured using 51Cr-labelled E.G7-OVA (•), or EL4 (○). The results shown are the per cent specific lysis at various dilutions of effector cells. One representative experiment of three is shown.