Figure 3.
In vitro production of IFN-γ by lung cells from the TcCMVIL-18 DNA-injected mice following MAC infection. Mice were i.m. injected with the TcCMVIL-18 or TcCMV DNA, or not injected. One day later, the mice were intranasally infected with 105 MAC. Single cell suspensions of lung cells were stimulated in vitro with 1 µg/ml Con A. Culture supernatants were collected after 72 hr and assayed for IFN-γ by sandwich ELISA. The values represent the mean±standard deviations of triplicate determinations from three mice killed at each time point. The data shown are representative of two similar experiments. The asterisk indicates statistically significant differences (P < 0·01) compared with the groups injected with the TcCMV DNA at each time point.