Table 2.
Effect of IL-18 DNA vaccination on NO production by lung cell cultures following MAC infection
| NO2− level (μm) | ||
|---|---|---|
| Treatment* | 3 week infected | 8 week infected |
| Uninfected | < 2·0 | < 2·0 |
| Intact infected | 35·2 ± 4·8 | 58·8 ± 1·3 |
| TcCMV (100 µg) | 29·8 ± 6·0 | 60·4 ± 5·2 |
| (300 µg) | 41·3 ± 2·8 | 66·7 ± 4·9 |
| TcCMVIL-18 (100 µg) | 113·7 ± 7·2† | 210·5 ± 13·7† |
| TcCMVIL-18 (300 µg) | 128·3 ± 5·7† | 207·2 ± 18·8† |
*
Mice were i.m. injected with the TcCMVIL-18 or TcCMV DNA, or not injected. One day later, the mice were intranasally infected with 105 MAC. Cultures of 2 × 105 lung cells were incubated for 72 hr in 200 µl volumes in a 96-well plate. Culture supernatants were harvested and assayed for nitrate levels. Data are the means±standard deviations of triplicate determinations from cultures pooled from five mice infected for 3 or 8 weeks. Experiments were repeated twice with similar results.
†
P < 0·001, relative to groups injected with the TcCMV DNA.