Skip to main content
. 2001 Mar;102(3):289–300. doi: 10.1046/j.1365-2567.2001.01196.x

Figure 2.

Figure 2

(a) Diagrammatic illustrations of the transgene constructs and cytoplasmic domains of the mutant IL-5Rα used in this study. All the transgene constructs have the wt IL-5Rα extracellular domain. Deletion mutants (DC3); bars represent deleted amino acid residues. Substitution mutants (ApvA); amino acid residues indicated by bold letters have been replaced with alanine. The cDNA encoding wt or the mutated IL-5Rα were ligated with the Eµ enhancer and VH promoter and used for preparing transgenic mice. (b) Expression of IL-5Rα on spleen B cells. Spleen cells from 8-week-old mice were stained with FITC-conjugated RA3-6B2 (anti-B220 mAb) and biotinylated T21 (anti-IL-5Rα mAb) followed by SA-PE. Profiles for IL-5Rα expression of B220+ cells are shown (shaded histograms). The solid line represents the histogram of IL-5Rα–/– B cells.