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. 2001 Mar;102(3):359–364. doi: 10.1046/j.1365-2567.2001.01192.x

Figure 4.

Figure 4

IgM binds to apoptotic HUVEC. HUVEC were cultured overnight in complete EGM (normal) or EGM without serum or growth factors (starved). Cells were incubated with 10% NHS or GVB++ (buffer) for 30 min at 37°, washed and stained with FITC-anti-IgM and PI. PI-positive cells were excluded from the analysis. Representative histograms of normal (a) and starved (b) cells incubated with NHS (dark line) or buffer (grey line) and stained with FITC-anti-IgM compared to unstained cells (shaded histogram).