Table 1.
Proliferative responses of splenocytes from BALB/c mice immunized with β-gal alone, or β-gal with adjuvant onto bare skin
| Immunization procedure | ||||||||
|---|---|---|---|---|---|---|---|---|
| β-gal | β-gal + LT | β-gal + CpG | β-gal + LT + CpG | |||||
| β-gal (μg/ml) | Mean c.p.m.±SD | SI | Mean c.p.m.±SD | SI | Mean cpm±SD | SI | Mean c.p.m.±SD | SI |
| 0.5 | 1600±153 | 1.7 | 2510±618 | 3.8 | 760±73 | 1.3 | 4104±490 | 14 |
| 0.05 | 1100±181 | 1.2 | 3036±391 | 4.6 | 789±220 | 1.4 | 7400±892 | 25.3 |
| 0.005 | 961±58 | 1 | 1738±357 | 2.6 | 915±202 | 1.6 | 4274±790 | 14.6 |
| Medium | 947±199 | 665±26 | 569±214 | 292±175 | ||||
Splenocytes were cultured in the presence of increasing concentrations of β-gal, and thymidine uptake was measured after 5 days culture. The data are presented as c.p.m. ± sd from hexaplicate cultures and as SI.