Table 1.
OVA-primed LN cells* | ||||
---|---|---|---|---|
OVA | Con A | |||
Cytokine† | 4-day | 8-day | 4-day | 8-day |
IL-4 (pg/ml)‡ | 33·1 ± 2·8 | < 4·9 | 87·3 ± 6·2 | 23·4 ± 2·0 |
IL-10 (U/ml)‡ | 16·1 ± 0·5 | 16·8 ± 1·1 | 31·0 ± 0·5 | 16·3 ± 0·3 |
IFN-γ (ng/ml)‡ | 9·5 ± 0·9 | 16·4 ± 2·7 | 21·5 ± 2·5 | 28·3 ± 1·2 |
IL-2 (ng/ml)§ | 1·6 ± 0·3 | 1·2 ± 0·2 | 14·4 ± 1·5 | 24·3 ± 1·4 |
*Culture supernatants from 4-day or 8-day OVA-primed LN cells in vitro restimulated with OVA (500 µg/ml) or Con A (5 µg/ml).
All the cytokines were assayed by two-site sandwich ELISA and the results represent the mean of duplicate cultures ±SD. The cytokine levels of unstimulated cultures were: IL-4 < 4·9; IL-10 < 3·1; IFN-γ < 1·5; IL-2 < 0·2.
IL-4, IL-10 and IFN-γ were measured in 72 hr (6 × 106 cells) supernatants.
IL-2 was quantified in 24 hr (10 × 106 cells) supernatants.