Table 1.

Cytokines secreted by lymph node cells from 4-day or 8-day OVA-immunized mice

	OVA-primed LN cells*
	OVA		Con A
Cytokine†	4-day	8-day	4-day	8-day
IL-4 (pg/ml)‡	33·1 ± 2·8	< 4·9	87·3 ± 6·2	23·4 ± 2·0
IL-10 (U/ml)‡	16·1 ± 0·5	16·8 ± 1·1	31·0 ± 0·5	16·3 ± 0·3
IFN-γ (ng/ml)‡	9·5 ± 0·9	16·4 ± 2·7	21·5 ± 2·5	28·3 ± 1·2
IL-2 (ng/ml)§	1·6 ± 0·3	1·2 ± 0·2	14·4 ± 1·5	24·3 ± 1·4

*Culture supernatants from 4-day or 8-day OVA-primed LN cells in vitro restimulated with OVA (500 µg/ml) or Con A (5 µg/ml).

^†All the cytokines were assayed by two-site sandwich ELISA and the results represent the mean of duplicate cultures ±SD. The cytokine levels of unstimulated cultures were: IL-4 < 4·9; IL-10 < 3·1; IFN-γ < 1·5; IL-2 < 0·2.

^‡IL-4, IL-10 and IFN-γ were measured in 72 hr (6 × 10⁶ cells) supernatants.

^§IL-2 was quantified in 24 hr (10 × 10⁶ cells) supernatants.