Figure 3.

Generation of NK1.1^– CD3^int cells by positive selection of the mutation on polymorphic MHC antigens. (a) Two-colour staining for CD3 and IL-2Rβ. (b) Two-colour staining for CD3 and NK1.1. B6 and B6-nu/nu mice were used at the age of 10 weeks as controls. B6-nu/nu mice at the age of 8 weeks were irradiated (4 Gy) and were injected with 10⁷ bone marrow cells of bm 1 or bm 12 origin. Immunofluorescence tests of lymphocytes in the liver, spleen and bone marrow (BM) were performed on day 14 after BMT. The data shown here are representative of five experiments.