Figure 4.

Five-day primary mixed leucocyte reaction (MLR) showing allogeneic T-lymphocyte activation in response to monocyte-derived dendritic cells (MoDCs). Proliferation was determined by [³H]thymidine incorporation. Represented are the means and SD of triplicate wells containing mitomycin-treated allogeneic MoDCs or peripheral blood mononuclear cells (PBMC) and 2 × 10⁵ fresh responding PBMC, at the indicated ratios. MoDCs or PBMC alone were included as controls. Data shown are representative of four experiments. IP, index of proliferation (see Materials and methods).