Figure 6.

Endogenous HEL and OVA are processed in different pathways. COS cells were transfected with A^k and varying amount of vector or HEL cDNA (a), or A^k/Ii and varying amount of vector or OVA cDNA (b), and 16 hr later were treated with or without 150 µm chloroquine for 6 hr. Presentation to KZO (a) or KZH (b) T cells was measured by induced lacZ activity. Data for HEL are representative of three independent experiments; data for OVA are representative of two independent experiments. neg., no antigen cDNA.