Figure 7.

Influence of deletions in the lumenal portion of Ii on presentation of OVA/A^k presentation to T cells. COS cells were transfected with A^kα + A^kβ plus vector or the indicated Ii construct. Cells were tested for presentation of varying concentration of synthetic OVA247–265 peptide (a), exogenously added native OVA protein (b), or endogenously synthesized OVA (c), to KZO T cells following overnight culture. T-cell activation was measured by induction of lacZ activity. Data in (a) are representative of two independent experiments; (b) and (c) are representative of three independent experiments. neg., no peptide (a), OVA (b), or OVA cDNA (c).