Figure 3.

(a) Brefeldin A does not inhibit the secretion of K^b-loading peptides. RMA-S and IC21 macrophage co-cultures were treated with 1 µg/ml of BFA for a period of 18 hr. RMA-S controls were treated with BFA as well. K^b expression on harvested RMA-S cells was analysed as described in Fig. 1. The histograms show RMA-S stained with an isotype-control antibody (Ic), K^b expression on RMA-S cultured alone (S) and K^b expression on the gated population of RMA-S cells from co-culture with non-infected macrophages (S-Non-Inf-Mφ) and with infected macrophages (S-Inf-Mφ). (b) K^b expression was also determined on the IC21 macrophages.