Table 2. Only phagocytic secrete peptides that load MHC class I molecules.
Kb expression on RMA-S | |||
---|---|---|---|
Co-culture | I | Y3 | Y3–I |
RMA-S | 2·28 | 18·04 | 15·76 |
RMA-S + l929 | 3·4 | 13·02 | 9·62 |
RMA-S + infected L929 | 3·05 | 14·14 | 11·09 |
RMA-S peritoneal macrophages | 4·15 | 33·19 | 29·04 |
RMA-S + infected peritoneal macrophages | 3·89 | 45·35 | 41·46 |
RMA-S + J774 | 2·95 | 48·2 | 45·25 |
RMA-S + infected J774 | 2·67 | 66·31 | 63·64 |
RMA-S | 7·98 | 30·59 | 22·61 |
RMA-S + A20 | 9·41 | 29·08 | 19·67 |
RMA-S + infected A20 | 4·13 | 28·94 | 24·81 |
Results are shown as the mean fluorescence channel. Co-cultures were performed as described in Fig. 1. L929, peritoneal macrophages from BALB/c mice, J774 and A20 were treated with IFN-γ for 48 hr and infected with Salmonella typhimurium at ratio of 100 bacteria per cell. I, isotype control antibody; Y3, antibody against Kb molecules.