Table 1.
Proliferation responses of the patient's PBMNC
Stimulation | Normal control (c.p.m.) | Patient (c.p.m.) |
---|---|---|
(–) | 1080 ± 15 | 1250 ± 12 |
PHA | 39780 ± 785 | 1578 ± 25 |
PMA + ionomycin | 62570 ± 1428 | 47850 ± 875 |
Anti-CD3 | 33150 ± 658 | 1625 ± 22 |
Ovalbumin | NT | 1440 ± 87 |
Ovomucoid | NT | 1329 ± 54 |
The patient's PBMNC were stimulated with PHA (5 µg/ml), PMA (10 ng/ml) and ionomycin (1 µg/ml), biotinylated anti-CD3 (100 ng/ml) plus streptavidin, ovalbumin (25 µg/ml), or ovomucoid (25 µg/ml) for 3 days. Cell proliferation was assessed by incorporation of 3H-thymidine. Each value represents the mean value for three experiments ±SEM. NT, not tested.