Table 2.
IL-4 production by PBMNC and CD4+ T cells
| PBMNC (pg/ml) | CD4+ T cells (pg/ml) | |||
|---|---|---|---|---|
| Stimulation | Control | Patient | Control | Patient |
| (–) | < 2·0 | < 2·0 | < 2·0 | < 2·0 |
| PMA + ionomycin | 2542 ± 123 | 2854 ± 151 | 3745 ± 873 | 2538 ± 1025 |
| Anti-CD3 | 1423 ± 151 | 583 ± 54 | 2523 ± 622 | 1785 ± 211 |
| Ovalbumin | NT | 85 ± 12 | NT | NT |
| Ovomucoid | NT | 73 ± 18 | NT | NT |
| Anti-CD3 + | ||||
| Piceatannol | NT | NT | 2055 ± 855 | 173 ± 98 |
The patient's PBMNC and purified CD4+ T cells were stimulated with PMA (10 ng/ml) and ionomycin (1 µg/ml), biotinylated anti-CD3 (100 ng/ml) plus streptavidin, ovalbumin (25 µg/ml), or ovomucoid (25 µg/ml) in the presence or absence of 10 µm piceatannol for 2 days. The amount of IL-4 in culture supernatants was measured by ELISA. Each value represents the mean value for three experiments ±SEM. NT, Not tested.