Table 1.

Serum IL-12 p40/70 levels in mice injected with synthetic and natural DNA

	SRA−/− mice	BALB/c mice
PBS	25 ± 10	10 ± 6
CpG ODN	255 ± 45*†	133 ± 46*
Control ODN	7 ± 4	4 ± 3
EC DNA	291 ± 197*	146 ± 109*
CT DNA	61 ± 48	14 ± 9

Groups of SRA^−/− and BALB/c mice (n = 3) were injected via their tail veins with 100 µl/mouse of PBS containing 20 µg of CpG-oligodeoxynucleotide (CpG ODN) or 200 µg of E. coli DNA (EC DNA). Blood was collected 4 hr after injection and serum IL-12 p40/70 levels were assessed by ELISA. Control mice were injected with 100 µl/mouse of PBS or 100 µl/mouse of PBS containing 20 µg of non-CpG-oligodeoxynucleotide (control ODN) or 200 µg of calf thymus DNA (CT DNA). The data represent one of three independent experiments. Results are represented as the mean±SEM.

^*P < 0·05 when compared with PBS group.

^†P < 0·05 when compared between SRA^−/− and BALB/c mice.