Figure 2.

IL-6 neutralization reduces cell proliferation and IL-2 production by T cells during a primary vaccination. C57Bl/6 mice were immunized as indicated in Fig. 1. Popliteal and inguinal lymph nodes were collected on day five and lymph node cells were cultured in vitro with (shaded bars) or without (open bars) the antigen (ST-CF). Cell proliferation was assayed 48 hours after stimulation (a) and IL-2 was measured in the cell culture supernatants 24 hours after stimulation (b). The results are expressed as mean±standard deviation of the results obtained per group. Statistically significant effects of the antibody treatment are labelled *(P < 0·05), **(P < 0·01), according to Student's t-test. NI, non-immunized; I, immunized.