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. 2001 Jul;103(3):375–381. doi: 10.1046/j.1365-2567.2001.01244.x

Figure 4.

Figure 4

Neutralization of IL-6 during a secondary vaccination stimulates both the IFN-γ and the IL-4 responses. C57Bl/6 animals (four per group) were injected in the hind footpads with PBS in DDA (open squares) or were immunized with ST-CF in DDA (circles) twice with a two weeks interval. Half of the immune mice were injected i.p. with a monoclonal antibody specific for IL-6 (open circles) 2 hr before the second immunization, while the other half received an irrelevant control immunoglobulin (closed circles). Popliteal and inguinal lymph nodes were collected on days 1, 2 and 4 and lymph node cells were cultured in vitro with (closed lines) or without (hatched lines) the antigen (ST-CF). The numbers of IFN-γ (a) and IL-4 (b) secreting cells was determined by the ELISPOT technique. Statistically significant effects of the antibody treatment at each time point are labelled **(P < 0·01), according to Student's t-test.