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. 2001 Jul;103(3):390–398. doi: 10.1046/j.1365-2567.2001.01249.x

Figure 1.

Figure 1

Characterization of bacterial strains. (a) Aliquots of strains M1, 12 and 57 GAS were incubated with normal human plasma, and bound proteins were eluted and analysed by SDS–PAGE and Western blotting using polyclonal anti-C4bp. The position of the 70 000 MW α-chain of C4bp is indicated by the arrow. Only strain M1 bound C4bp. (b) Aliquots of overnight culture supernatant from strains M1, M12 and M57 GAS were analysed by SDS-PAGE and Western blotting using polyclonal anti-M1 SIC. This showed that SIC is produced by strains M1 and M57 but not by M12. (c) Southern blot of HindIII digested streptococcal DNA from strains M1, 12 and 57 probed with labelled M1 sic PCR amplicon showing the presence of a sic or sic-like gene in all three GAS strains tested. No hybridizing bands were detected in either of the negative control Streptococci (data not shown).