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. 2001 Jul;103(3):390–398. doi: 10.1046/j.1365-2567.2001.01249.x

Figure 4.

Figure 4

SIC binds to C5b-7, C5b-8 and C5b-9 complexes in ELISA. ELISA plates were coated with SIC (panels a and b), rSIC (panels c and d) or coating buffer only and intermediate or complete TCC complexes were added. Binding was detected with (i) polyclonal anti-C6 (a and c); or (ii) monoclonal anti-C5b neo-epitope (b and d). SIC and rSIC bind preferentially to C5b-7 complexes and to a lesser extent to C5b-8 and C5b-9 using anti-C6 to detect, but predominantly to C5b-8 using anti-C5b neo-epitope. Results displayed are the net ODs after deduction of background in coated wells plus antibodies but no sera.