Figure 2.

(a) Effect of GVHD on the number of spleen cells, intestinal IEL and hepatic lymphocytes. On day 14 after induction of GVHD, spleen cells, intestinal IEL and hepatic lymphocytes were prepared as described in the Materials and Methods. The mean±SD for four mice is shown. *P < 0·05, **P < 0·01. (b) Engraftment of donor T cells in the target organs. The number of engrafted donor T cells was determined by multiplying the total number of the cells by the fraction of H-2K^d-negative and CD3-positive cells (acute GVHD) or by the fraction of H-2K^b-negative and CD3-positive cells (chronic GVHD). The mean±SD for four mice is shown.