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Expansion of host splenic B cells and NK1.1+ T cells in mice with chronic GVHD. Spleen cells and hepatic lymphocytes were prepared from normal BDF1 mice and chronic GVHD mice as described in the Materials and Methods, and two-colour staining was performed for B220 and H-2Kb, or NK1.1 and H-2Kb, respectively. The numbers in the figure represent the percentage of fluorescence-positive cells in the corresponding areas.
