Figure 7.

Influence of modulating lysosomal functions on the infection/replication of ASFV in monocytic cells. (a) Adherent monocytic cells were either untreated controls (unfilled histogram), or LPS-treated (filled histogram). In certain LPS-treated cultures, the cells received the lysosomal enzyme substrates MetOMe or GPN, as described in the Materials and methods, followed by infection with ASFV (MOI 1 IFFU/cell). (b) Monocytes were cultured for only 24 hr before treatment with the lysosomal substrates and infection with ASFV, as in (a). Analyses were performed at 24 hr p.i. for the production of the de novo ASFV-specific late structural protein (VP73). Antigen-positive cells, detected with the anti-VP73 mAb, were determined by gating on the cells to the right of the negative antibody control peak, as for Figure 2. The results are expressed as means ± SEM from five replicate experiments.