Figure 3.

Analysis by two-dimensional gel electrophoresis of Mycobacterium tuberculosis-infected THP1 cells. The experiment is identical to that described in Figure 2, except that a linear pH 4–7 IEF first-dimension and 7·5% second-dimension gel was used. The up-regulated protein shown in the insert was found to be the ATP synthase β chain, the product of the M. tuberculosis atpD gene (c).