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. 2001 Oct;104(2):127–134. doi: 10.1046/j.0019-2805.2001.01308.x

Figure 4.

Figure 4

Flow cytometric analysis of B220+ 5-and-6-carboxyfluorescein diacetate, succinimidyl ester (CFDA)-labelled and B220+ endogenous spleen cells 6 weeks after transfer of CFDA-labelled normal spleen cells into IL-2−/− and IL-2+/+ (C57BL/6) mice. B220 (FL3) and CFDA (FL1) were detected on spleen cells from a representative IL-2−/− recipient (a) and from a representative IL-2+/+ (C57BL/6) recipient (b). Values represent the % of B220+/CFDA+ cells (upper right) and B220−/CFDA+ cells (lower right). In (c), values represent the mean ± SE of the % of B220+ cells of the total CFDA-labelled cells detected in spleens of IL-2−/− (n = 3) and IL-2+/+ (C57BL/6) (n = 3) mice. Values in (d) represent the mean ± SE of endogenous B220+ cells in spleens of the same mice. Values in (e) represent the mean ± SE of the % of CFDA+ cells of the total spleen cells from the same mice.