Table 1.

B-cell and total cell numbers in the bone marrow and spleen of IL-2^−/− mice ^*

		Source of cells
Cell subpopulation	Age (weeks)	IL-2−/−	IL-2+/+
Bone marrow
B220+/IgM−	8–10†	1·7 ± 1·1‡	4·1 ± 1·6
	11–13	1·3 ± 0·6	4·4 ± 0·1
	22–26	0·5 ± 0·1	3·7 ± 0·9
Total cells	8–10†	26·5 ± 8·9	19·4 ± 6·2
	11–13	35·9 ± 13·2	21·8 ± 4·5
	22–26	10·7 ± 3·9	20·2 ± 5·4
Spleen
B220+	8–10	26·7 ± 11·3	57·7 ± 26·5
	11–13	4·3 ± 2·3	46·7 ± 18·4
Total cells	8–10†	142·6 ± 54·0	105·9 ± 49·1
	11–13	110·6 ± 2·3	100·0 ± 31·6

^*Bone marrow and spleen cells were collected from IL-2^−/− and IL-2^+/+ mice between the ages of 8 and 26 weeks. Cell subpopulations were identified by flow cytometry.

^†Number of mice per group. IL-2^−/−: 8–10 weeks, n = 8; 11–13 weeks, n = 3; 22–26 weeks, n = 2. IL-2^+/+: 8–10 weeks, n = 7; 11–13 weeks, n = 2; 22–26 weeks, n = 5.

^‡Values represent the mean ± SD of the number of cells(×10⁶) in each subpopulation determined by multiplying the % of bone marrow B220⁺/IgM⁻ cells (bone marrow gate includes lymphocytes and large granular cells) or splenic B220⁺ cells (spleen gate includes lymphocytes only) by the total number of cells obtained from bone marrow (femurs) or spleen of each animal.