Figure 3.

Intravenous CsA inhibits the late phase but not the early phase of IgE-mediated hypersensitivity. BALB/c mice were passively sensitized on day 0 by i.v. injection of monoclonal anti-TNP IgE antibody in varying doses (a) 200 µg, (b) 20 µg, and (c) 2 µg. Then, 24 hr later, mice were challenged on the ears with 0·8% PCl and ear thickness was measured at various times later. A separate group of mice that received identical doses of IgE were treated i.v daily with 1 mg CsA in saline on day −1 to day +1. A negative control group was not immunized, nor treated with CsA, and received saline vehicle alone, and then were identically ear challenged with PCl to provide background swelling responses, that were then subtracted from the responses of the experimental groups, to derive the net swelling responses shown (note different scale of y-axis in each part of the figure).