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. 2001 Oct;104(2):142–148. doi: 10.1046/j.0019-2805.2001.01285.x

Figure 1.

Figure 1

Generation of ACAID correlates with a small but significant increase in the percentage of γδ T cells in the spleen. Spleen cells from normal B6 or B6 mice injected with 50 µg of OVA in the anterior chamber 7 days earlier were stained with FITC-conjugated anti-CD3, PE-conjugated anti-TCR δ-chain and Cy-chrome-conjugated anti-TCR β-chain. Lymphocytes were gated based on forward angle and side light scatter, then CD3+ cells were gated and 250 000 events were collected. Dual colour staining of a representative mouse for each group is illustrated on a log scale. Nine spleens per group were analysed individually using three-colour flow cytometry and the percentages of αβ and γδ T subsets in CD3+ cell population are indicated as average (mean±SD) of each group. P values were calculated using Students t-test.