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RT–PCR analysis of mature Cε mRNA.BALB/c spleen cells were cultured with or without 1 µg/ml RMCP-I in the presence of LPS plus IL-4 for 4 days. cDNA was reverse transcribed from mRNA, and amplified with the primer pairs for mature Cε (a) and β-actin (b) for indicated cycles. Bands in agarose gel were visualized with ethidium bromide.
