Figure 2.

Purified CD8αβ⁺ αβ and γδ T cells differentially modulate CD8β after activation. CD4-depleted splenocytes were purified by magnetic-activated cell sorting (MACS) and activated by panning on T-cell receptor (TCR)-coated plates in the presence of 0·1 µg/ml of CD28 monoclonal antibody (mAb). After 24 hr, cells were washed and cultured in interleukin-2 (IL-2)-containing medium. Cells were analysed by three-colour flow cytometry for TCR, CD8αβ and CD8αα expression at the indicated time-points after initiation of the culture. The panels show cells electronically gated for expression of the respective TCR. Numbers in the quadrants represent the frequency of cells. The purity of γδ or αβ T cells was > 99%.